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《Journal of Anhui Agricultural Sciences》 2009-19
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Research on the Accurate Determination of Flavonoid in Three Pentaphyllum Varieties with Different Taste

SU Ding-chang et al(Key Laboratory of Biological Resources Protection and Utilization of Hubei Province,Hubei Institute for Nationalities,Enshi,Hubei 445000)  
[Objective] The accurate determination of flavonoid in new Pentaphyllum varieties,Enwuyemi and Enqiyetian,was conducted for the reference for its development and utilization.[Method] The original mutant of Pentaphyllum being taken as the control,the flavonoid in Enwuyemi and Enqiyetian in maturity stage and same growth environment and field management was extracted with cable-type devices.The concentration of test reagents,the ratio of material to liquid,the extraction time and temperature was optimal with orthogonal design.[Results] The best extraction combination with the orthogonal design was the ethanol concentration,70%;the ratio of material to liquid,1∶20;the extraction temperature,80 ℃ and the extraction time,2 hours.The content of the best combination of parameters of extraction process of original mutation,Enwuyemi and Enqiyetian was 2.19%,3.65% and 3.58%.[Conclusion] The extract of flavonoid from sweet Pentaphyllum had industrial value.The flavonoid from the Pentaphyllum original mutant was very bitter in taste and the extract of flavonoid from sweet Pentaphyllum,Enwuyemi and Enqiyetian,was sweet in taste,which was with better value in the development of functional health products.
【Fund】: 生物资源保护与利用湖北省重点实验室资助项目(2007022);; 湖北省教育厅高校产学研合作重点资助项目(2009)
【CateGory Index】: R284.1
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