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《Journal of Anhui Agricultural Sciences》 2009-19
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Study on the Extraction and Activity of Superoxide Dismutase from Vatica numgachampoi

LIU Jian-ping et al(College of Life Sciences,Chongqing Normal University,Chongqing 400047)  
[Objective] The research aimed to determine the activity of superoxide dismutase(SOD)in Vatica numgachampoi and screen out the most effective method of extracting crude SOD from V.numgachampoi.[Method] With V.numgachampoi from Qijiang of Chongqing as raw materials,Different material-liquid ratio was used to extract crude SOD from V.numgachampoi.Highly purified samples of SOD were obtained by ammonium sulfate precipitation and Sevage method.The detection results of the activity,protein content and specific activity of crude SOD obtained from different steps were compared.[Result] The dosage of H3PO4 buffer had direct influences on the extraction of crude SOD.When the volume ratio of H3PO4 buffer to V.numgachampoi was 3∶1,the specific activity of SOD was highest(25.34 U/mg protein).When the mixed solution of chloroform and ethanol was added to crude enzyme solution at the proportion of 3∶1,the protein removal effect was obvious.When the saturation of added(NH4)2SO4 to crude enzyme solution reached 90%,the precipitation effect of SOD was better and the specific activity was increased 51.43 U/mg protein than the extract.[Conclusion] The optimum extraction conditions of crude SOD from V.numgachampoi were as follows: the volume ratio of H3PO4 buffer to V.numgachampoi of 3∶1,at 25-30 ℃,pH value of 7.8,removing protein by using Sevage method and the saturation of(NH4)2SO4 for precipitating SOD increased from 35% to 90%.Under these conditions,total protein was 215.13 mg,total enzyme activity was 5 415.74 U/mg protein,the specific activity was 25.34 U/mg protein.
【Fund】: 重庆市扶贫办科技扶贫项目资助
【CateGory Index】: R284.1
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