Genetic Diversity Analysis of 44 Shares of Hibiscus cannabinus L.Germplasm Resources Using ISSR Molecular Marker
HUO Guang et al(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha,Hunan 410205)
[Objective] This study was to reveal the genetic diversity and genetic relationship of the kenaf(Hibiscus cannabinus L.) resources from different origins,thus providing basis for genetic improvement and molecular marker-assisted breeding of kenaf.[Method] Ninety one ISSR molecular markers were used for amplification on 44 shares of kenaf germplasm resources,of which 21 that showed good diversity and clear bands were chosen for PCR amplification.Based on amplification results,the genetic similarity coefficients among kenaf germplasm resources were calculated by using analytic software NTSYSpc-2.10e,and phylogenetic tree was then established via UPGMA.[Result] Totally 169 bands were amplified using the 21 screened primers,averagely 8.05 bands were amplified from each primer.Of them,141 bands were polymorphic,accounting for 83.4%.When genetic similarity coefficient 0.887 was used as criterion L1,these 44 shares of kenaf germplasm could be classified to be 32 shares of cultivars and 12 shares of wild type or half-wild type varieties.When genetic similarity coefficient 0.897 was used as criterion L2,these 32 shares 32 shares of cultivars could be further grouped into four sub-clusters.The genetic diversities between cultivars and wild type or half-wild type varieties were 0.47-0.91,showing huge hereditary difference;while that among 32 cultivars were 0.85-0.97,suggesting that genetic relationships among cultivars are relatively close and their genetic similarities were rather narrow.[Conclusion] ISSR could well determine the genetic similarities among kenaf germplasm resources and provide valuable molecular information for selecting parents of hybrid cross,which can lay a good foundation for DNA mapping of kenaf germplasm resources.