Isolation and Identification of Dairy Cattle Brucellosis
LI Ji-lin(Animal Husbandry and Veterinary Station of Ledou County,Ledou,Qinghai 810700)
[Objective] The basis of the isolation and identification of dairy cattle brucellosis was provided.[Method] The investigation of the cattle disease caused by brucellosis was conducted.A dairy farm survey was made,After collection of serum,blood,milk and other samples the hydrogen sulfide test,test,and thermal dye inhibition agglutination test were conducted for brucellosis isolation and identification.[Results] The 21 milk samples in 10% CO2 concentration in the culture incubator to 4 days,5 growth medium of 30-50,0.05-2.00 mm Size colorless,flashing round,moist,small colony of the suspicious bulge.The results showed that hydrogen sulfide produced,five strains may have a hydrogen sulfide;dye bacteriostasis test results show that indeed for the five strains of brucellosis;heat agglutination test results show that the 5 strains did not mutate;PCR test results show that the,corresponding to the milk tested positive.[Conclusion] cloth from the initial identification of disease-positive cattle suspected isolated five strains for bovine brucellosis.