Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anhui Agricultural Sciences》 2009-24
Add to Favorite Get Latest Update

The Orthogonal Design of Chinese Angelica Polysaccharide with Water Extraction and the Optimum Process of Removing Protein with Method of Savag's

YANG Jing et al (Institute of Traditional Chinese Veterinary Medicine,Nanjing Agricultural University,Nanjing,Jiangsu 210095)  
[Objective] The study aimed to select the best process of Chinese Angelica Polysaccharide(CAPS) with water extraction and the optimum operating times of removing protein with method of Savag's.[Method] The orthogonal design was used to extract of CAPS.The Savag's method was used to remove protein from polysaccharide and the polysaccharide contents were compared after the different removing times.The total polysaccharide contents were determined with method of phenol-sulfuric acid,the protein content determined by method of Coomassie brilliant blue,the uronic acid content with method of sulfate-carbazole.[Result] The optimum extracting process was that ratio of material to water was 1∶20,the extraction time was 30 minutes,extraction temperature was 100 ℃ and the optimum process of removing protein was 3 times(5∶1 ratio of chloroform:n-butanol).[Conclusion] The method was simple and easy to operate and was suitable for extracting and detecting of water-soluble polysaccharide.
【Fund】: 南京农业大学本科生SRT项目(0704A04);; 南京农业大学校青年科技创新基金(KJ07015);; 教育部科学技术重点项目基金(E200803)
【CateGory Index】: S567.239
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 8 Hits
1 ZHANG Qing-yong;YU Hong;WANG Qing-kui;SUN Xue-liang;XING Ke-zhi;GUO Yong-jun;Tianjin Key Laboratory of Aqua-Ecology and Aquaculture;;Extraction,Separation and Purification of Angelica sinensis Polysaccharides[J];河北渔业;2016-03
2 WU Jianjun;SHAO Jing;WU Jiandong;LI Hongdi;LI Wenjun;ZHANG Yan;LIU Yongqi;WU Hongyan;Key Laboratory for Research of Molecular Medicine and TCM Prevention and Treatment on Major Diseases of Gansu Colleges and Universities;Key Laboratory for Chinese Herbs Exploitation,Innovation and Transformation;Institute of TCM Preventive Medicine,School of Public Health of Gansu University of Chinese Medicine;;Study on the optimization of the extraction technology for Angelica sinensis polysaccharide based on orthogonal design[J];甘肃中医学院学报;2015-05
3 NIU Xianli;JI Keping;ZHONG Jingjun;WEI Nina;PENG Liping;Zhuhai Campus,Zunyi Medical College;;Technology Optimization of Extracting Polysaccharide from Radix trichosanthis by Cellulose Enzyme[J];贵州农业科学;2014-07
4 XIE Hong;CAO Ye;LIU Jing;YIN Mei;Shen Yang Agricultural University;;Optimization of extraction process of water-soluble polysaccharides from seeds meal of ginseng[J];食品工业科技;2014-09
5 ZHANG Su-bin;LYU Jia-xin;College of Chemistry and Chemical Engineering,Zhaoqing University;;Comparison on Four Extraction Methods of Polysaccharide from Angelica Sinensis[J];天津农业科学;2013-10
6 LI Jia-lin et al(Gannan Medical University,Ganzhou,Jiangxi 341000);Study on Microwave-assisted Extraction of Polysaccharide from Angelica sinensis[J];安徽农业科学;2012-34
7 LI Shao-hong1,XIA Peng-fei2,MA Xiao2,SHAO Huan2(1.Department of Pharmacy of Wuxi No.2 People′s Hospital in Jiangsu,Wuxi 214002,China;2.Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province,Lanzhou 730000,china);Study on Quality Evaluation of Angelica sinensis by Grey Incidence Degree Method[J];中药材;2012-11
8 YUE Hong-kun,JIA Hui-zhen,WANG Jun College of Chemical Technology,Shijiazhuang University,Shijiazhuang,Hebei 050035,China;The Extraction Technology of the Volatile Oil in Angelica sinensis(Oliv.) Diels Based on Supercritical CO_2 Extraction[J];Medicinal Plant;2010-04
【Citations】
Chinese Journal Full-text Database 2 Hits
1 YANG Tie-hong, JIA Min, MEI Qi-bing~*(Department of Pharmacology, Fourth Military Medical University, Xi’an 710032, China);Effect of Angelica sinensis polysaccharide fraction AP-3 on IL-2 and IFN-γ induction[J];药学学报;2006-01
2 Guo Yazhen, Wang Zao (College of Food Science, SFU, 200090) Qu Pengpeng (Laboratory of Food Package and Technology, Shanghai Food Research Institute, 200233);Purification and identification of polysaccharide from Lentinus edodes[J];上海水产大学学报;2000-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SHAO Jian-bing;LIU Jian;WANG Yan;XIA Guo-hua;XIE Bing;Department of Pharmacy,Rugao People's Hospital;School of Pharmacy,Jiangsu University;Department of Laboratory,Zhenjiang Dantu Hospital of Traditional Chinese Medicine;;Purification technology of total phenolic acid from angelica[J];实用药物与临床;2016-11
2 ZHANG Yuejin;LIU Xianyun;CHEN Xiangmin;TIAN Xiaojuan;LI Wenshu;XUE Xiangyang;School of Nursing, Wenzhou Medical University;Pharmacy of Traditional Chinese Medicine, the First Affiliated Hospital of Wenzhou Medical University;Biological Laboratory Education Center, Wenzhou Medical University;Institute of Molecular Virology and Immunology, Department of Microbiology and Immunology, Wenzhou Medical University;;Construction and immune response of recombinant whole-cell yeast expressing HCMV-UL138 CTL epitope[J];温州医科大学学报;2015-06
3 LAO Xuefen;ZOU Hui;XU Xiaomeng;WANG Chengkai;CAO Zheng;XU Xiaoqin;Coll of Vet Med/Jiangsu Co-Innov Cent for Prev and Cont of Imp Anim Infect Dis and Zoonoses,Yangzhou Univ;;Effect of water extract and polysaccharides from Se ligustrum lucidum on immunity in mice[J];扬州大学学报(农业与生命科学版);2015-01
4 Zheng Xiaoke;Yang Meng;Bai Yiping;Guo Yueting;Zhang Minghui;Kuang Haixue;Feng Weisheng;School of Pharmacy, Henan University of Traditional Chinese Medicine;Collaborative Innovation Center for Respiratory Disease Diagnosis And Treatment & Chinese Medicine Development of Henan Province;School of Pharmacy, Heilongjiang University of Chinese Medicine;;Experimental Study on Immunomodulatory Effect of Ethanol Sediments of the seeds of Descurainia sophia(L.) Webb. ex Prantl.[J];世界科学技术-中医药现代化;2015-03
5 Chen Xiangmin;Zhang Yuejin;Tian Xiaojuan;Xia Ping;Pan Weiwen;Xia Tian;Yu Chenhui;Zhang Li fang;Xue Xiangyang;The Biological Laboratory Education Center,Wenzhou Medical University;;Construction of recombinant yeast expressing PreS2_(120-146) and hepatitis B surface antigen and evaluation of the immune effects of whole yeast cells[J];中华传染病杂志;2014-11
6 YUAN Ju-li;LIU Feng-lin;Gansu University of Traditional Chinese,Teaching Experiment Center;;Orthogonal extraction process of angelica polysaccharides[J];应用化工;2014-10
7 GUO Shu-zhen;ZHANG Xiao-xiang;LEI Hong;School of Medical Engineering, Hefei University of Technology;School of Biological and Food Engineering, Hefei University of Technology;;Removing Proteins from the Grifola frondosa Polysaccharide by Sevage Method[J];安徽化工;2014-05
8 Lu Ling;Zhang Lanmei;Wu Jun;Ren Li;Liu Guo;Hemodialysis Center,Nuclear Industry 215 Hospital of Shanxi Province;;Clinical effects of treating 72 patients with chronic renal failure and hemodialysis by warming kidney and invigorating spleen[J];国际中医中药杂志;2014-08
9 SHI Lai-bao;CHEN Jing;YIN Xia;Reyoung Pharmaceutical Co. ,Ltd.;;Study on the extraction methods of lentinan[J];药学研究;2014-04
10 CHEN Hong-shuo;LI Xiao-ying;FENG Peng-mian;BAI Song;GAO Jian-sheng;College of Electrical Engineering,HeBei United University;College of Public Health,HeBei United University;Jitang College of HeBei United University;;Study on Anti-tumor Effect of Spirulina Polysaccharide on H22 in Mice[J];食品研究与开发;2014-05
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Cheng-peng;LIU Jun;JIA Dao-yong;ZHANG Meng-si;XU Chun-yan;ZHANG Yan-yan;JING Peng-wei;WANG Lu;WANG Shun-he;WANG Ya-ping;Laboratory of Stem Cell and Tissue Engineering,Chongqing Medical University;Department of Pathology,Chongqing Medical University;Department of Histology and Embryology,Chongqing Medical University;;Study on mechanism of telomere system regulation and senescence induction of leukemia stem cells by Angelica sinensis polysaccharide[J];中草药;2014-16
2 LI Jia-lin et al(Gannan Medical University,Ganzhou,Jiangxi 341000);Study on Microwave-assisted Extraction of Polysaccharide from Angelica sinensis[J];安徽农业科学;2012-34
3 WANG Nuo-zhou,ZHANG Yang-de(Tongji Medical College,Huazhong University of Science and Technology, Wuhan,Hubei 430030,P.R.China);The pharmacological effects and research progress of angelica polysaccharide[J];中国现代医学杂志;2012-19
4 ZHANG Xin-guo,WANG Qiang-lin,LI Chun-lei,ZHANG Ying-peng,LI Kun, HU Shu-juan,REN Jing,ZHOU Li,ZHI Kang-kang,DAI Xing-chao(Lanzhou University of Technology,Lanzhou 730050,China);Enzymatic Extraction of Polysaccharide from Angelica[J];中医药学报;2012-03
5 GENG Wei-pu 1,XU Man 1,LUO Yi 2,WANG Ting-ting 1,HUANG Wen-lian 1,CHEN Yu 1(1.Dept of Pathology,Molecular Medicine and Tumor Research Center of Chongqing Medical University,2.Dept of Obstetrics and Gynecology,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China);The promotional effect of Ganoderma lucidum polysaccharide and Angelica sinensis polysaccharide on the proliferation and IFN-γ secretion of human peripheral blood T lymphocytes[J];中国药理学通报;2012-05
6 YU Yang-ding1,2,3 QI Hang1,2,3 LI Dong-mei1,2,3 HE Yao-bin1,2,3 BI Jing-ran1,2,3(1.School of Food Science and Technology,Dalian Polytechnic University,Dalian,Liaoning 116034,China;2.Engineering Research Center of Seafood in Ministry of Education,Dalian,Liaoning 116034,China;3.Liaoning Province Key Laboratory of Seafood Science and Technology,Dalian,Liaoning 116034,China);Optimization on pectase-assistant extracting technological conditions of polysaccharide from sporophyll of Undaria pinnatifida by response surface methodology[J];食品与机械;2012-01
7 ZHANG Li 1,FAN Ming-cai 1,FENG Xi-wen2,LIU Xing-yong2,WEI Ran2(1.School of Chemistry and Pharmaceutical Engineering,Sichuan University of Science & Engineering,Zigong 643000,China; 2.School of Material and Chemical Engineering,Sichuan University of Science & Engineering,Zigong 643000,China);Study on the extraction of dendrobium candidum polysaccharides and dendrobine by cellulase[J];化学研究与应用;2011-03
8 LI Feng1,LIU Chun-li2,ZHANG Zhen-qiu1,LI Ke-qiang1,3,QIAO Ge4,LIU Jian-ping1(1.Liaoning University of Traditional Chinese Medicine,Dalian 116600,Liaoning,China;2.Shengyang Dougyu Pharmaceutical Co,IED,shengyang 110036,Liaoning,China;3.institute of Traditional Chinese Medicine Liaoning Province,Shenyang 100163,China,4.Liaoning Provincial Government Procurement Center);Studies on Quality Evaluation of Bombyx Batryticatus by Grey Indidence Degree Method[J];辽宁中医杂志;2011-02
9 WANG Ming-dao,HU Hong,GAO Feng,LI Su-zhen,CHEN Hong-ge*(College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China);Optimization of Microwave Extraction of Polysaccharide from Ranunculus ternatus[J];江西农业学报;2011-01
10 LI Hong-rui*,YAO Shu-lin,CHEN Yun-chao,ZOU Li-qiang College of Food Science and Engineering,Jiangxi Agricultural University,Nanchang,Jiangxi 330045,China;Optimization of Extraction Process of RHIZOMA DIOSCOREAE Polysaccharide and Determination of Its Antioxidant Activity[J];Medicinal Plant;2010-12
【Secondary References】
Chinese Journal Full-text Database 9 Hits
1 Zhang Rui;Pharmacy Department,Shangluo Central Hospital;;Quality Evaluation of Radix Bupleuri Fructus from Different Habitats by Grey Correlation Analysis and Principal Components Analysis[J];中国药师;2017-08
2 Wang Lihua;Wang Feifei;Liu Yue;Bi Ye;Zhang Xinfang;Zhao Jing;Ling Ningsheng;Tianjin Vovational College of Bioengineering;;Review on polysaccharide from Angelica sinensis (Oliv) Diels[J];天津药学;2017-03
3 GONG Yu-hong;LUO Guang-ming;ZHANG Feng-bo;YUAN Yuan-jian;LI Bo;XIAO Ri-chuan;XIE Jing;ZHANG Bo-wen;School of Pharmacy,Jiangxi University of Traditional Chinese Medicine;;Quality Evaluation of Gardeniae Fructus by Grey Incidence Degree Method[J];中国实验方剂学杂志;2017-13
4 WANG Jie;ZHU Shun-juan;FAN Qin;YANG Li;XIA Peng-fei;WANG Yin-quan;ZHAO Lei;Gansu University of Chinese Medicine;Key Laboratory for Traditional Chinese (Tibetan) Medicine Chemistry and Quality Control of Gansu University;Key Laboratory of Pharmacology and Toxicology of TCM of Gansu Province;Northwest Collaborative Innovation Center for Chinese Medicine;Cultivation Base for Key Laboratory of Chinese Medicine Quality and Standard of Gansu Province;;Quality Evaluation of Gentiana officinalis H.Smith. from Different Producing Areas of Gansu Province by Grey Incidence Degree Method[J];中国中医药信息杂志;2017-03
5 CHEN Shi-tao;ZHA Qin;ZHANG Jun;CHEN Sha;LIU An;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;Jiangxi University of Traditional Chinese Medicine;Bijie Institute of Chinese Materia Medica;;Application of Dynamic Optimization Method in Extraction Process Optimization of Ginseng Radix et Rhizoma[J];中国实验方剂学杂志;2017-04
6 WANG Ming-wei;LI Shuo;LI Min;LI Cheng-yi;YANG Fu-de;Gansu University of Traditional Chinese Medicine ( TCM);Chengdu University of TCM;;Comprehensive Evaluation on Medicinal Quality of Angelica Sinensis Radix of Different Cultivars( Strains) by Entropy TOPSIS Model[J];中国实验方剂学杂志;2017-05
7 LI Cheng-yi;QIANG Zheng-ze;WANG Yan;LI Shuo;WANG Ming-wei;Department of Pharmacy,Gansu College of Traditional Chinese Medicine;;Quality Evaluation of Hedysari Radix by Grey Incidence Degree Method[J];中国实验方剂学杂志;2016-12
8 WANG Ying;ZHANG Shang-zhi;ZHU Tian-tian;JIN Ling;LI Ying-dong;Department of Biochemistry, Dingxi Teachers College;Gansu University of Chinese Medicine;Research Institute of Chinese (Tibetan) Medicinal Resources, Gansu University of Chinese Medicine;;Researches on the extraction and analysis of effective components of Angelica sinensis[J];中兽医医药杂志;2016-02
9 LI Cheng-yi;LIU Shu-bin;LI Shuo;WANG Ming-wei;LI Jun-yue;ZHANG Ying-shan;Gansu University of Chinese Medicine;Gansu Province Engineering Research Institute of Modern Pharmaceutical of Traditional Chinese Medicine;;Quality Evaluation of Merchandise Baitiao Codonopsis Radix in Gansu Province by Grey Relational Analysis[J];中国实验方剂学杂志;2015-21
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 WU WU-TONG YU PIN-HUA XIA ER-NING SHI WEI-XING XU BI-RU CHEN QIONG-HUA Nanjing College of Pharmacy;ISOLATION AND PURIFICATION OF POLYSACCHARIDES TF-A,TF-B AND TF-C FROM THE SPORES OF TREMELLA FUCIFORMIS BERK AND DETERMINATION OF THEIR MOLAR RATIOS[J];Acta Biochimica et Biophysica Sinica;1984-04
2 Fan Huizeng, Chen Judi ahd Lin Kezhong (Institute of Materia Medica, Tientsin);AN ACIDIC MUCOPOLYSACCHARIDE ISOLATED FROM STICHOPUS JAPONICUS SELENKA AND SOME OF ITS PHYSICAL AND CHEMICAL PROPERTIES[J];药学学报;1980-05
3 Ji Minhou;Xu Zuhong;Guo Yucai Institute of Oceanlogy,Academia Sinica;A STUDY ON THE FUCOIDAN OF LAMINARIA JAPONICA[J];水产学报;1980-01
4 XIE ER-NING CHEN QIONG-HUA(Division of Biochemistry,China Pharmaceutical University,Nanjing);ISOLATION,PURIFICATION AND IDENTIFICATION OF THE POLYSACCHARIDE FRON AURICULARIA AURICULA UNDERW[J];Acta Biochimica et Biophysica Sinica;1988-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved