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《Journal of Anhui Agricultural Sciences》 2009-24
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Simultaneous Determination of Chlorogenic Acid,Rutin and Luteolin in Flos Lonicerae by RP-HPLC

HAO Feng-xia et al (Key Laboratory of Energy Sources and Chemical Engineering,Ningxia University,Yinchuan,Ningxia 750021)  
[Objective] The aim of the study was to determine chlorogenic acid,rutin and luteolin contents in Flos Lonicerae simultaneously.[Method] With Zorbax SB-C18 column(250 mm×4.6 mm,5 μm),methanol-0.02% Phosphoric acid(50∶50)as mobile phase,flow velocity at 1.0 ml/min,detection wave length at 350 nm and column temperature at 30 ℃,the chlorogenic acid and the flavonoid compounds contents in Flos Lonicerae were determined by RP-HPLC.[Result] The chlorogenic acid,rutin and luteolin contents have obvious differences in different kinds of Flos Lonicerae.[Conclusion] The method is simple,feasible,and can be used to control the quality of Flos Lonicerae.
【Fund】: 宁夏大学科学研究基金项目(ZR200706 ZR200807)
【CateGory Index】: R284.1
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