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《Journal of Anhui Agricultural Sciences》 2009-25
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Study on the Tissue Culture of Amorphophallus albus

GUO Zheng-hong et al (Biotechnical Center of Three Gorges University,Yichang,Hubei 443002)  
[Objective] The study aimed to screen the media suitable to induction,differentiation and rooting of Amorphophallus albus.[Method] With the corm and scale of A.albus in Zigui County in Hubei Province as tested material,MS was used as the basic medium with different hormones to respectively match 10 kinds of media for induction,differentiation for tissue culture of A.albus The induced clumpy buds were cut into single plants and were put into the rooting medium of MS+NAA 0.1-0.5 mg/L and the effects of different hormone matching on callus formation,bud differentiation and rooting were studied.[Result] The corm and scale were easy to induce the callus on the medium of MS+1.0 mg/L 6-BA+1.0 mg/L NAA,with the induction of 92% and 90% resp.and the induced callus was easy to differentiate.The corm had the differentiation rate of 86.7% on the medium of MS+1.5 mg/L 6-BA+0.15 mg/L NAA and the scale had the differentiation rate of 83.3% on medium of MS+2.0 mg/L 6-BA+0.2 mg/L NAA.When the adventitious buds differentiated by corm and scale were transferred into the rooting medium of MS+0.5 mg/L NAA,the rooting reached to 94% and the whole plants were obtained after culture for 20 d.[Conclusion] The regenerative system of A.albus was established preliminarily in the experiment,which provided good technique support for producing A.albus seedlings in great scope.
【Fund】: 国家自然科学基金(30871579)
【CateGory Index】: S632.9
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