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《Journal of Anhui Agricultural Sciences》 2009-26
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Effect of Hormone Treatment on in Vitro Propagation of Gerbera jamesonii Bolus

ZHOU Heng et al(Department of Biological Engineering,Tongren Vocational and Technical College,Tongren,Guizhou 554300)  
[Objective] The aim was to study the effect of different hormone treatments on in vitro propagation of Gerbera jamesonii Bolus.[Method] With the double-flower cut-flower cultivar of G.jamesonii Bolus Sangren as tested materials,MS was used as the basic medium and was added by different concn.of BA,KT,NAA and IAA.The differentiation and induction,subculture and proliferation as well as rooting culture tests were carried out on G.jamesonii Bolus so as to research the effect of different hormone types,combination and concn.on induction and differentiation,subculture and proliferation as well as rooting culture of G.jamesonii Bolus and screen the best combination of hormones.[Result] The induction effect with IAA 0.1 mg/L + BA 10 mg/L treatment on buds was best and the bud differentiation rate was the highest in initial culture;the proliferation effect with IAA 0.1 mg/L + BA 1.0 mg/L treatment on buds was best in subculture,on which the bud seedlings grew tidy,leaves were dark green and shoots were over 2 cm in height;the effect with IBA 0.01 mg/L treatment was the best at rooting stage,on which the number of produced root was moderate and suitable for the growth of seedlings.[Conclusion] The appropriate combination and proportion of cytokinin and auxin played an important role in callus induction and bud differentiation of G.jamesonii Bolus.
【Fund】: 铜仁职业技术学院教授工程项目资助
【CateGory Index】: S682.11
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