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《Journal of Anhui Agricultural Sciences》 2009-26
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Optimization of Extraction Conditions for Oxidation Resistant Components from Pogadatree Flower and Qualitative Analysis

ZHU Yu-yan et al(Beijing Key Laboratory of Plant Resources Research and Development,School of Chemistry and Environment Engineering Beijing Technology and Business University,Beijing 100048)  
[Objective] The paper aimed to study the optimize extraction conditions for antioxidant substance production from pogadatree flower and its qualitative analysis.[Method] The optimize extraction conditions of total oxidation resistant components,total phenols and total flavonoids of pogadatree flower(Sophora japonica L.) were determined by orthogonal test.[Result] The result showed that the optimal condition of pogadatree flower extracted by the means of total antioxidant ability was determined as 70% ethanol when the extraction time was 2 h without pretreatment.The best extraction condition for total phenol substances was identified as 70% ethanol with 0 h of pretreatment and 2 h of extraction.In contrast,the optimized condition for total flavonoids was determined as follows: 50% ethanol,pretreatment time 6 h,extraction time 3 h.The intuitive analysis results indicated that extraction solvent was the main factor that effected on the active substances of pogadatree flower extracts in total antioxidant ability,phenol and flavonoides.The correlation analysis showed that antioxidant ability of pogadatree flower contained some other bioactive substances besides the total phenol and flavonoids.[Conclusion] The research provided the theoretical basis for applying the extraction from pogadatree flower in anti-aging food.
【Fund】: 北京市科技新星项目(2007B011)
【CateGory Index】: TS202
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