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《Journal of Anhui Agricultural Sciences》 2009-32
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Study on Establishment of cpSSR Marker Technology and Optimization of Its Reaction System in Jatropha curcas Linn

XIANG Qian et al (College of Forestry and Horticulture,Sichuan Agricultural University,Yaan,Sichuan 625014)  
[Objective] The aim was to build an optimal cpSSR-PCR system for Jatropha curcas Linn.[Method] Optimize cpSSR-PCR amplification system for Jatropha curcas Linn in terms of five factors(Taq DNA polymerase,Mg 2+,DNA template,dNTP and primer)from several levels.[Result] An optimal cpSSR-PCR system for Jatropha curcas Linn was obtained as 2.00 mmol/L Mg 2+,2 U/μl Taq DNApolymerase,0.2 mmol/L dNTP,0.2 μmol/L Primer,35 ng/μl DNA template for 20 μl reaction syetem.[Conclusion] The optimal annealing temperature for cpSSR-PCR reaction syetem is determined as 52 ℃.The cpSSR reaction system was steady and reproducible.
【CateGory Index】: S565.9
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