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《Journal of Anhui Agricultural Sciences》 2009-33
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Research on the Protein Extracted from Chlorella with the Method of Thermal Steam-NaOH

JIANG Huai-zhen et al(College of Food Science and Engineering,China Ocean University,Qingdao,Shandong 266003)  
[Objective] The process conditions of the protein extracted from chlorella with the method of thermal steam-NaOH were optimized through the study on its extraction method.[Method] The effect of the steam pressure,extraction time,ratio of solid-liquid(algae powder-water)and amount of adding NaOH(the percentage in algae powder)on the rate of protein extracted from chlorella was experimented after the chlorella cells were treated with thermal steam and solution of salt or alkali together.[Results] The results showed that the combing treatment of thermal steam and alkali solution was more conducive to the dissolution of chlorella protein and more than 90% of chlorella protein may be alkali-soluble protein.The best extraction condition of optimized chlorella protein was:the amount of adding NaOH,20%;the ratio of solid-liquid,1∶50;the extraction time,45 minutes and the steam pressure,0.075 MPa;under which condition,the rate of protein extracted from chlorella was 91.19%.[Conclusion] The combing treatment of thermal steam and alkali solution could significantly increase the rate of protein extraction from chlorella cells.
【Fund】: 中国科学院知识创新工程重要方向项目(KSCX2-YW-G-060)
【CateGory Index】: Q946
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