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《Journal of Anhui Agricultural Sciences》 2009-33
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Influences of Different Culture Conditions on the Secondary Metabolites in Dendrobium loddigesii Calli

LU Wen-yun et al(College of Chemistry and Environmental Science,Guizhou University for Nationalities,Guiyang,Guizhou 550025)  
[Objective]The research aimed to explore the relationship between the growth of Dendrobium loddigesii and its main secondary metabolites-the dendrobine and the accumulation of dendrobium polysaccharides.[Method]The phenol-concentrated sulphuric acid colorimetry and bromocresol green acid dye colorimetry were used to determine the effects of different culture conditions on polysaccharides and total alkaloids in the callus of D.loddigesii.[Result]The growth curve of callus of D.loddigesii in suspension culture was "S" type.Under the culturing condition of light,the accumulation of polysaccharides and total alkaloids in the callus of D.loddigesii in suspension culture was basically synchronized with its growth.The light was beneficial for the growth of callus in suspension culture and the accumulation of polysaccharides.But under the condition of dark,the callus grew slowly and the accumulation of polysaccharides was not synchronized with its growth.But the dark culture could enhance the accumulation of total alkaloids greatly.[Conclusion]There were a close relationship between the growth of D.loddigesii and its main secondary metabolites-the dendrobine and the accumulation of dendrobium polysaccharides and the different culture conditions had some influences on the secondary metabolite of D.loddigesii.
【Fund】: 贵州省教育厅自然科学类科研项目(黔教科2007052)
【CateGory Index】: S567.239
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