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《Journal of Anhui Agricultural Sciences》 2009-33
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Experiment in the Pathogenicity of Isaria farinosa to Lipaphis erysimi

CHEN Zhen et al(School of Protection Biology,Southwest Forestry College,Kunming,Yunnan 650224)  
[Objective] The reference for the effective biological control method of Lipaphis erysimi was provided through the research on the pathogenicity of Isaria farinosa to it.[Method] The Isaria farinosa conidia washed from a PDA plate with sterile water,which suspension with high concentration of 1.5×108 spores/ml was prepared for the test of aphid-infected.The test in toxicity to Lipaphis erysimi was conducted with 5 treatments with concentrations of 1.5×104(pf1),1.5×105(pf2),1.5×106(pf3),1.5×107(pf4)and 1.5×108(pf5)spores/ml.[Results] The infection rate of aphid reached 92.22%,the mortality rate was 100% and the mortality rate corrected was 83.33% after the treatment of 1.5×108 spores/ml for 7 days,which indicated the Isaria farinosa strain(PFCB001)was easy to infect the aphid with had high virulence.Paecilomyces farinosus on the virulence Diaeretiella The regression equation of the pathogenicity of Isaria farinosa to Lipaphis erysimi was:y=0.595 7x-3.324 0 and LC50 was 3.799 0×105 spores/ml,and with the concentration-increasing the mortality rate of aphid was also increased;the longer the treatment time was,the higher its mortality rate was.[Conclusion] The Isaria farinosa had good pathogenicity to Lipaphis erysimi and it had the great potential in control of Lipaphis erysimi.
【Fund】: 昆明市科学技术局资助项目(200201008)
【CateGory Index】: S476.1
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