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《Journal of Anhui Agricultural Sciences》 2009-33
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Preparation of Hydrogen Peroxide Biosensor without Intermedium by Electric Potential Controlling of Hb

XIE Yi et al(Analysis and Testing Center,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101)  
Hemoglobin was immobilized on L-cysteine modified gold electrode by using the method of electric potential controlling to develop a new hydrogen peroxide biosensor Hb/L-cys/Au.The modified process of electrode was characterized by the means of CV and AFM.The results showed that the sensor had excellent catalysed reduction performance to H2O2,high sensitivity and good stability.And it was easy to be produced.This electrode presented an excellent electrochemical response for the determination of H2O2 concentration with high sensitivity in 0.1 mol/L PBS(pH value of 5.5).The linear scope could be divided into 2 sections of concentration section(8.21×10-8-4.83×10-6 mol/L)and low concentration section(4.83×10-6-8.22×10-3 mol/L).The detection limit was 8.24×10-9 mol/L.
【CateGory Index】: TP212.3
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