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《Journal of Anhui Agricultural Sciences》 2009-35
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ISSR Analysis on Five Hyriopsis cumingii Populations in Jiangsu Aera

CHEN Chan-juan et al (College of Life Science,Nanjing University,Nanjing,Jiangsu 210093)  
[Objective] The study aimed to analysis the genetic diversity and genetic relationship of 5 Hyriopsis cumingii populations in Jiangsu aera by using Inter-Simple Sequence Repeat(ISSR) technique.[Method] The genome DNA of five H.cumingii populations were amplified by using 10 ISSR primers in which the five primers with more amplification bands,strong signal and clear background were selected to make the amplification analysis on 5 populations from Taihu in Wuxi City(TH),Hongzehu(HZH),Jiangpu in Nanjing(JP),Xinghua 1(XH1),Xinghua 2(XH2) which was 121 individuals in total.[Result] The 70 DNA fragments were obtained and their sizes were from 200 to 2 000 bp through ISSR amplification,in which,the 61 sites were polymorphism and the proportion of polymorphic site was 87.14%.The gene diversity index of 5 populations were 0.259 9-0.314 4 with the average of 0.290 1 and the Shannon message index was 0.392 2-0.466 3.The highest value for 2 kinds of the genetic diversity index in breed populations in JP were 0.314 4 and 0.466 3 resp.,but the lowest value for that of wildness populations in TH were 0.259 9 and 0.392 2 resp.[Conclusion] The gene diversity of H.cumingii breed populations was higher than the wildness populations and the genetic relationship of 5 H.cumingii populations had no significant correlation with the geography position.
【Fund】: 江苏淡水珍珠蚌种质资源保护与利用(BM2006703)
【CateGory Index】: S917.4
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