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《Journal of Anhui Agricultural Sciences》 2009-35
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Somatic Embryogenesis from the Leaves of Rosa rugosa in vitro Culture

YANG Lu-jun et al (Zhengzhou Institute of Agricultural and Forestry Sciences,Zhengzhou,Henan 450005)  
[Objective] The study was to provide the fundamental material for transgenic research of Rosa rugosa.[Method] The leaves of Rosa rugosa plantlets were used as explants and cultured in MS basic medium.The effects of different concentrations of three kinds of plant growth regulators(6-BA,Ad,NAA) and their combinations on somatic embryogenesis were studied in vitro culture.Meanwhile,the influences of different concentrations of sucrose on somatic embryogenesis were studied.[Result] The induction effects of cytokinins 6-BA was better than Ad in embryoid induction.The auxin NAA combined with given concentrations of 6-BA and Ad could effectively raise the frequency of somatic embryogenesis.The optimum plant growth regulator combination for somatic embryogenesis was 0.4 mg/L 6-BA+0.2 mg/L Ad+0.2 mg/L NAA.20-30 g/L sucrose added in the medium was beneficial to somatic embryogenesis.The results of histological observations indicated that somatic embryos originated from upper epidermis and mesophyll histiocyte single cell nearby stoma.[Conclusion] The optimum medium for the somatic embryogenesis from leaf explants of Rosa rugosa was 1/2 MS+0.4 mg/L 6-BA+0.2 mg/L Ad+0.2 mg/L NAA+20-30 g/L sucrose.
【Fund】: 河南省科技厅重点攻关项目(0623010500)
【CateGory Index】: S685.12
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【Citations】
Chinese Journal Full-text Database 2 Hits
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2 LI Mei ru 1, LI Hong qing 2, SUN Zi jian 1, CHEN Yi zhu 1 ( 1. South China Institute of Botany, Chinese Academy of Sciences, Guangzhou 510650, China; 2. Life College, South China Normal University , Guangzhou 510631, China );Advance in tissue culture and genetic transformation of rose[J];Guihaia;2003-03
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