Study on the Total Flavonoids of Humulus scandens(Lour.) Merr.Technology in DM130 Macroporous Resin Concentration
WU Guan-zhong et al(Chinese Pharmaceutical University,Nanjing,Jiangsu 210009)
[Objective]To study the technology for purification of flavonoids from Humulus scandens(Lour.) Merr.by DM130 macroporus adsorbtion resin and determine the content of cosmosiin,vitexin and luteolin by Gradient HPLC.[Method]The proper technology parameters of purification of flavonoids were obtained through single factor test.Chromatographic separation was peformed in Hanbon Kromasil C_8 column (4.6 mm×250.0 mm,5.0μm) at 25℃with an molibe phaseA consisting of CH_3OH-H_2O-CH_3COOH(30:70:0.1) and molibe phaseB of CH_3 OH-CH_3 COOH(1 000:1) at a flow rate of 1.0 ml/min,the molibe phase was monitored by a photodiode array detector at 350 nm.[Result] The best technical parameter of DM130 macroporous resin were as follows,the content flavonoids was 0.2 mg/ml,adsorption flow rate was 2.0 BV/h,pH value was 6,after eluted with 4 BV distilled water,the eluting regent 3.5 BV 50%ethanol and eluting rate 2.0 BV/h. The linear range of vitexin was 0.16 - 20.00μg /ml,the linear range of cosmosiin was 0.16 - 20.00μg /ml,and the linear range of luteolin was 0.16 - 20.00μg /ml.Luteolin had a good linear relationship with peak area in the 0.40 -50.00 ug/ml concentration range.[Conclusion] The DM130 macroporus adorsption resin showed better comprehensive adsorption property,and the average recovery of the method was 88%,the contents of vitexin,luteolin and cosmosiin in each elution fraction group were different.
【CateGory Index】： R284