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《Journal of Anhui Agricultural Sciences》 2009-36
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Effects of Several Chemical Factors on the Sperm Activity of Misgurnus anguillicaudatus

CHAI Yi et al(College of Animal Science,Yangtze University,Jingzhou,Hubei 434025)  
[Objective]The aim was to discuss the activities of Misgurnus anguillicaudatus sperms in solutions of K~+,Na ~+,Ca~(2+) and Mg~(2+) at different concn.and ascertain the optimum concn.parameters of physicochemical factors of activation solution for M.anguillicaudatus sperms.[Method]The sperms were collected by squeezing method and the effects of several chemical factors on the activity of M.anguillicaudatus sperms were studied through observing and comparing their activities in different tested solutions.[Result]The activity of M.anguillicaudatus sperms in KCl solution reached highest when its concn.was 0.5%,being 246 s and then it was decreased sharply along with the increase of the concn.The activity of M.anguillicaudatus sperms in NaCl solution reached highest when its concn.was 0.4%,being 100 s which was lower than the highest activity of sperms in KCl solution,and then it was decreased rapidly along with the increase of the concn.of NaCl solution.The activity of M.anguillicaudatus sperms in CaCl_2 solution reached highest when its concn.was 0.4%,being 27 s and that in MgCl_2 solution reached highest when its concn.was 0.3%,being 20 s,and then the sperm activities showed slow decreasing trend in both the two solutions along with the increase of their concn.[Conclusion]In the artificial fertilization process of fishes,the use of water with Ca~(2+) and Mg~(2+) at high concn.should be avoided in preparing activation solutions for sperms.
【CateGory Index】: S917.4
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