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《Journal of Anhui Agricultural Sciences》 2010-03
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Research on Extraction of Diosgenin through Itself Transformation in Dioscorea zingiberensis C.H.Wright

WANG Ying et al(College of Biology and Food Engineering,Dalian Industry University,Dalian,Liaoning 116034)  
[Objective] The aim was to research the extraction method of diosgenin through itself transforming in Dioscorea zingiberensis C.H.Wright.[Method] D.zingiberensis was preserved for 15 and 30 d resp.under the room temperature condition on base of the effect of itself enzyme in D.zingiberensis And then the diosgenin with 3 glycosyl was part transformed into non-glycosyl diosgenin under the effect of itself enzyme,the itself transformed diosgenin in D.zingiberensis was extracted,separated and measured and the extraction effect under the different preserve times were compared.[Result] When 500g D.Zingiberensis were preserved for 15 and 30 d under the room temperature resp.,the diosgenin were got by 30.8 and 24.7 g resp.and the total saponin yield was 6.16 % and 4.94 % resp.through extracting by 70% ethanol;the diosgenin was got by 6.3 and 11.7 g resp.and the saponin yield was 1.26% and 2.34% after separation and purification by using macroporous absorption resin;and then the final diosgenin was 4.97 and 10.2g and its yield was 0.99% and 2.04% after decoloration by using D-280 macroporous absorption resin.[Conclusion] The diogenin in D.zingiberensis preserved at 30 d was 2 times of that preserved at 15 d after separation and purification by using macroporous absorption resin,and its purity was 91% by HPLC detection.
【Fund】: 国家自然科学基金资助项目(30470055);; 辽宁省教育厅创新团队项目(2007T006)
【CateGory Index】: S567.239
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