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《Journal of Anhui Agricultural Sciences》 2010-04
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Determination of Several Hydrolases Related to Immune Function in the Process of Artemia Development

ZOU Guang-zhong et al(College of Life Sciences,Ludong University,Yantai,Shandong 264025)  
[Objective]The reference for the study on the immunology of Artemia was provided through the test of several hydrolases related to immune during the Artemia.[Method]The activity of the acid phosphatase(ACPase),alkaline phosphatase(AKPase) and α-naphthyl acetate esterase(ANAE) of a certain number of Artemia bodies,including eggs,nauplius,metanauplius,mimetic imago,and imago stage,which were hatched in laboratory in March-May of 2009,were determined with the method of benzene disodium phosphate,Kim method and spectrophotometer determination,respectively.[Results]The activity of three kinds of enzymes in Artemia development was gradually increased from the egg,nauplius,to metanauplius stage,then decreased from the metanauplius to the mimetic imago stage and finally increased again and reached to maximum in the imago stage.[Conclusion]The study showed that the activity of three kinds of enzymes was directly correlated with the immune of Artemia,which could be used as measurable indicator of the Artemia immune function.
【CateGory Index】: S963.214
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