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Study on Molecular Phylogeny to the Species of Anisoptera (Insecta:Odonata) Based on CO Ⅱ Complete Genes

ZHANG Xiao-Jie et al (Department of Laboratory Medicine,Bengbu Medical College,Bengbu,Anhui 233000)  
[Objective] The research aimed to study the molecular phylogeny to the species of Anisoptera(Insecta:Odonata) based on CO Ⅱ complete genes.[Method] Non-special primers were designed on the CO Ⅱ genes of Odonata.CO Ⅱ complete genes of 6 genera,including 8 species of 5 families in Anisoptera,had been sequenced and analyzed.The phylogenetic trees were reconstructed using CluxtalX1.8,ContigExpress,MEGA2.1,PHYLIP3.6a and MrBayes V3.0 sofewares,and maximum parsimony and maximum likelihood methods,respectively.[Result] The results show that A+T contents of CO Ⅱ genes of Libellulidae are lower(68.6%) in Insecta,which proves Odonata is an original group.Every sequence includes 2 Cys,which differs from the other groups of Insecta.Do not agree with the view of raising Macrominae into Macromidae.Agree with the views of putting both Aeshnidae and Cordulegastridae into Aeshnidea,and raising Gomphidae into a superfamily.Order of the evolution relationship of 5 families is:Gomphidae→Cordulegastridae→Aeshnidae→Corduliidae→Libellulidae.[Conclusion] The study can provide the relative basis for molecular phylogeny study of Odonata.
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