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《Journal of Anhui Agricultural Sciences》 2010-07
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Phylogenetic Analysis of Actinobacteria Isolates from Iron Mine Tailings

LV Zhi-tang et al(College of Life Science,Hebei University,Key Lab of Microbial Diversity Research and Application of Hebei Province,Baoding,Hebei 071002)  
[Objective] The aim was to discuss the actinobacteria biodiversity of iron-mine tailings by phylogenetic analysis of 12 typical isolates.[Method] The genomic DNAs were extracted by phenol-chloroform method;phylogeny analysis was carried out based on 16S rDNA PCR amplification and sequencing.[Result] The results showed that all the 12 strains belong to the genus Streptomyces sharing 98.7-99.9% similarities with their nearest known neighbors.[Conclusion] Streptomyces is the dominant culturable actinobacteria group of iron mine tailings,in which there are many potential novel species.
【Fund】: 国家自然科学基金项目(30800003);; 河北大学省基金预研项目(2006Y09)
【CateGory Index】: TD926.4
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