Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anhui Agricultural Sciences》 2010-08
Add to Favorite Get Latest Update

Research on the Method of Paraffin Section of the Corn Stalk in Its Maturity Stage

LI Su-kun et al (School of Plant Sciences and Technology,Beijing Agricultural College,Beijing 102206)  
[Objective] The method of paraffin section suitable for corn stalk in a maturity stage with structural characteristics such as high degree of lignification and fibrosis,high hardness materials,was established.[Method] The tissue between two internodes at basal part of maturity corn being taken as experimental material,s of the suitable method of paraffin section of corn stalk was formed through a series of the improvements in main linkage such as dehydration,transparent,transparent wax,embedding,staining,etc,based on the traditional method and combined with structural characteristics of corn stalk in maturity stage.[Results] The improved method shortened the production cycle as a whole compared with the traditional method;the time in linkage of dehydration was reduced because of the material containing low content of moisture.The application of the measure wax debris penetrating into material level by level,instead of traditional one,corn stalk paraffin method than of combined with corn stalk maturity to reduce dehydration time of the wax level-up the penetration can make thick-walled cells completely being filled in with wax.The dyeing cycle of material was shortened because of its structural characteristics of a high degree of lignification in the maturity stage.Thus,10μm cross-section slice of maturity corn stalk,with clearly staining and complete organizational structure,was prepared.[Conclusion] The basis of further anatomical observation and study on corn growth mechanism and the identification of variety was provided,while the technical reference for the paraffin section of the cereal plant stalk in maturity stage of monocotyledon was supplied.
【Fund】: 北京农业育种基础研究创新平台项目(D08070500690802)
【CateGory Index】: S513
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved