Effect of Herbicide on EST Isozyme Expression in Goldfish Serum
ZHANG Xiao-hong et al (Taiyuan Teachers College,Taiyuan,Shanxi 030031)
[Objective] The research aimed to explore the effects of herbicide on the expression of EST isozyme.[Method] Three kinds of herbicide(butachlor,paraquat,2,4-D butylate) were adopted,and 0.5 ml/L,1.0 ml/L,1.5 ml/L,2.0 ml/L concentrations each of them were prepared according 24 h LC50.Goldfish was injected with 0.1 ml/g dose.SDS-polyarylamidegel electrophoresis test was used to study the toxicity on expression of EST.[Result] Compared with the control,four concentration groups showed significant variety.The activity of EST reduced at low concentration,and the activity increased or disappeared at high concentration.Concentration and activity had dose-effect relationship.[Conclusion] Three kinds of herbicide had toxicity on EST isozymes.The activity of different herbicide was different.