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《Journal of Anhui Agricultural Sciences》 2010-10
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Extraction of Flavonoids Compounds in Chestnut and Identification on Its Composition

WANG Qian (Hebei Energy Institute of Vocation and Technology,Tang shan,Hebei,063004 )  
[Objective] To investigate the optimum extraction conditions of the flavonoids in Chestnut and the structure of flavonoids in Chestnut.[Methods] A series of single factor and orthogonal experimental design were adopted,the absorbance of the extracting solutions were determined,and the extraction ratio of flavonoids was taken as assessment indicators,to investigate the optimum extraction conditions of the flavonoids in Chestnut.Then the structure of flavonoids in chestnut were identified through color reagent method and spectrogram.[Results] The optimal extraction process of flavonoids in chestnut was showed as follows:solid-liquid ratio 1:50,temperature 60 ℃,extraction time 5 h,ethanol concentration 80%.The optimum extraction conditions,extraction rate of flavonoids was up to 0.51%.Flavonoids in chestnut were those which had 7-OH structure or ortho-dihydroxy structure at the A ring,such as flavones,flavonols,isoflavones alcohols,flavanones,Chalcones.[Conclusion] These results may provides a theoretical basis for the extraction process and structure identification of flavonoids in chestnut.
【CateGory Index】: S664.2
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