Study on Extraction Technology of Intracellular Polysaccharide from Byproduct of Hericium erinaceus by Ultrasonic Wave
CHAI Jun-hong et al (Department of Biology,MuDanJiang Teachers College,Mudanjiang,Heinongjiang 157012)
[Objective] To determine the optimum ultrasonic wave extraction technology of polysaccharide from byproduct of Hericium erinaceus. [Method] Polysaccharide was obtained by crushing,ultrasonic extraction,concentration,sevag method,and alcohol extraction,and polysaccharide contents were determined by the anthrone-sulfuric method. Based on the polysaccharide extraction process of optimization of single-factor test,using orthogonal test to determine the optimum extraction technology of intracellular polysaccharide from by product of Hericium erinaceus by ultrasonic wave.[Result] While pH value was from 5 to 6.5,the optimum technology of extracting polysaccharide from byproduct of Hericium erinaceus by ultrasonic method: solid-liquid ratio (volume ratio) was 1∶70; ultrasonic power was 80% of the maximum power;extraction time was 45 min,extraction temperature was 60 ℃,and the extraction rate of polysaccharide from byproduct of Hericium erinaceus was up to 9.56%. [Conclusion] Extraction technology of polysaccharide from byproduct of Hericium erinaceus had a certain degree of development and utilization value,it provided the theoretic basis for the comprehensive utilization of the wastes and byproduct of Hericium erinaceus.