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《Journal of Anhui Agricultural Sciences》 2010-11
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Study on Extraction Technological Conditions of Total Flavonoids from Toona sinensis Leaves

HUANG Hong-ying et al (Department of Chemistry and Life Science,Xiangnan University,Chenzhou,Hunan 423000)  
[Objective] The aim was to study extraction technological conditions of total flavonoids from Toona sinensis leaves in order to provide theoretical basis for further development of T. sinensis leaves in the field of food and medicine. [Method] Spectrophotometry was adopted to determine total flavonoids in T. sinensis leaves. By using single-factor test,effects of ethanol concentration,solid-liquid ratio,temperature and time on extraction rate of total flavonoids were analyzed. Based on this,the extraction technological conditions of total flavonoids from T. sinensis leaves were optimized through orthogonal design method. [Result] The results showed that the optimal extracting conditions were as follows:ethanol concentration 70%,solid-liquid ratio 1∶50,extracting duration 2.0 h and refluxing temperature 70 ℃. Under these optimum experimental conditions,total flavonoids yield from T. sinensis leaves could reach 5.84%. [Conclusion] The test was repeated for 5 times under the optimum extraction conditions,and the mean yield of total flavonoids was 5.84%.Therefore,the method is stable and reliable.
【Fund】: 湖南省教育厅科学研究基金项目(06C789)
【CateGory Index】: TQ461
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