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《Journal of Anhui Agricultural Sciences》 2010-14
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Study on Suspension Culture and Induction of Callus from Panax ginseng Embryos

YUE Cai-jun et al (College of Life Science and Biotechnology,Heilongjiang August First Land Reclamation University,Daqing,Heilongjiang 163319)  
[Objective]The research aimed to establish a method for suspension culture and induction of callus from Panax ginseng embryos,and investigate growth and ginsenoside accumulation of the suspension culture cells. [Method]The calli were induced from P. ginseng embryos in the MS medium added with 2. 4 -D (3 mg/L),NAA (2 mg/L) and 6-BA (0. 5 mg/L). Suspension cultures of the calli were established by putting the loose calli subcultured 8 times into liquid media with the same plant hormone as the media used to induce calli. The growth curve was described and six ginsenisides in the cells were analyzed by HPLC after the cells were subcultured 5 times. [Result]The induction rate of calli from the active embryos was 100%. The biomass of suspension cultures of the calli increased 1. 5 -fold in 21 -day cultivation. Six ginsenoside individ-uals were detected in the suspension cell cultures. Ginsenoside Rb1,Rg and Re in the cells were higher levels and 0. 44% ,0. 35% and 0. 37% of the dry cell weight,respectively. [Conclusion]Calli induction from P. ginseng embryos and suspension culture of the calli were easy. Suspension cultures of the calli grew fast and contained many ginsenoside individuals.
【Fund】: 黑龙江省普通高等学校青年学术骨干支持计划项目(1152G024)
【CateGory Index】: S567.51
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Chinese Journal Full-text Database 1 Hits
1 CHEN Wei1, GAO Wen-yuan1, JIA Wei2, DUAN Hong-quan1, XIAO Pei-gen3 (1.College of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China; 2.School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200030, China; 3.Institute of Medicinal Plant, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China);Advances in studies on tissue and cell culture in medicinal plants of Panax L.[J];Chinese Traditional and Herbal Drugs;2005-04
Chinese Journal Full-text Database 3 Hits
1 WANG Jian-hua,XUE Jian,HOU Chun-xi,XU Li-xin,ZHAO Shou-jing(College of Biological and Agricultural Engineering,Jilin University,Changchun 130022,China);Callus Induction in Hairy Roots of Panax ginseng[J];Journal of Jilin Agricultural University;2008-05
2 WANG Yi1,ZHAO Wen-jun1,YANG Zhong2,SUN Chun-Yu1,ZHANG Mei-ping1(1.School of Life Science,Jilin Agricultural University,Changchun 130118;2.Jilin Provincial Quality Supervision Council,130000 China);Studies on Somatic Embryogenesis and Plant Regeneration of Panax Ginseng[J];Pharmaceutical Biotechnology;2008-04
3 WANG Dan,WANG Zhen-yue,WANG Zong-quan,et alHeilongjiang University of TCM,pharmacy faculty,Harbin 150076,Chin;Brief on the progress of productive ways of the secondary metabolites in medicinal plants[J];Information on Traditional Chinese Medicine;2008-01
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