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Research Advances of Chromosome Micro-dissection Technology and Its Utilization in Plants

LIU Yu-ling et al(Cotton Research Institute,Chinese Academy of Agricultural Sciences / Key Laboratory of Cotton Genetic Improvement of Ministry of Agriculture,Anyang,Henan 455000)  
Chromosome micro-dissection technique is a direct bridge from cytogenetics research to molecular genetics research.It has been widely used in many fields.The principle of chromosome micro-dissection technique and the major procedures were reviewed.And some major technical problems such as chromosome identification,micro-dissection and micro-cloning were discussed and the application progress of this technique in plants was illustrated.
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