Effects of Extraction RNA from Soybean Root under Different Developmental Stages
ZHANG YANG et al(Jilin Agriculture University,Changchun,Jilin 130118)
[Objective] The aim was to study the best way to extract RNA from soybean root of different development stages and the extraction effects after flooding treatment.[Method]With Jinong18 as material,total root RNA under different developmental stages and culture conditions were extracted by RNAiso Reagent kit.The integrity and purity were examined by 2% denatured agarose gel electrophoresis and UV-spectrophotometer.[Result]The extraction of total RNA under sandy conditions was better than the soil condition,and the sample after flooding treatment was better than before treatment.[Conclusion]The root total RNA after flooding treatment under different development stages were satisfied for gene cloning and gene expression analysis requirement.
【CateGory Index】： S565.1