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《Journal of Anhui Agricultural Sciences》 2010-18
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Expression of a Tobacco Glycosyltransferase Gene's Promoter in Transgenic Tobacco

WANG Xue et al(College of Life Science,Key Lab for Biotechnology of the State Ethnic Affairs Commission,South-Central University for Nationalities,Wuhan,Hubei 430074)  
[Objective]The aim was to study the expression of the deletion fragments from the promoter of a glycosyltransferase gene induced both by MeJA and SA cloned from Tobacco W38(sm-Ngt) in the trangenic Tobacco.[Method] The T1 transgenic Tobacco seedling with the deletion fragments from the promoter of sm-Ngt and Gus gene was treated with MeJA and SA for 16 h,and then histochemical staining and fluorometric analysis of GUS activity of the transgenic plants T1 were carried out to determine GUS activity and analyze the effection of MeJA and SA on expression of the 5 deletion fragments from the promoter of sm-Ngt. [Result] In the 5 transgenic T1 Tobacco seedling with the different deletion fragments from the promoter of sm-Ngt,histochemical staining of GUS activity in the transgenic plant with-220-0 bp fragment was least,and with-524-0 and-468-0 bp fragments were the most.In the condition of untreated with MeJA and SA,the GUS activity in the transgenic tobacco seedlings growing 30 days with-524-0 and-468-0 bp were far outclass those with-1 150-0,-800-0 and-220-0 bp,furthermore,this was not because of the copy number.The GUS activity in the transgenic plants with-800-0 bp were induced by both MeJA and SA,and the-1 150-0 bp fragment was induced only by MeJA.[Conclusion]There was element exsited that enhancing the expression of sm-Ngtin-524——220 bp of the promoter,and restraining element in-1 150——524 bp region.And in the-1 150-524 bp region there existed many elements induced by both MeJA and SA.
【Fund】: 湖北省自然科学基金(2004ABA123)
【CateGory Index】: S572
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