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《Journal of Anhui Agricultural Sciences》 2010-19
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Rapid DNA Extraction Method from Amorphophallas. konjac and ISSR-PCR Amplification

CHEN Xue-yan(School of Biological Science and Engineering,Shaanxi Institute of Technology,Hanzhong,Shaanxi 723001)  
[Objective] The research aimed to study the total DNA extraction methods of Amorphophallas.konjac.[Method] To investigate the optimum DNA extraction method,CTAB,SDS and new modified methods for isolating genomic DNA from Amorphophallas.konjac leaf were studied and the isolated DNAs were examined by ultraviolet absorption test,agarose gelelectrophoresis and ISSR-PCR test for comparative analysis.[Result] The results showed that the new modified method was suitable for DNA isolation from Amorphophallas.konjac plants with high purity and yield;quality of DNA could meet ISSR-PCR requirement even extracted once by the new modified method.[Conclusion] The study provided basis for extracting high quality DNA sample,and offered the molecular genetics reference for exploring the genetic diversity of Amorphophallas.konjac.
【Fund】: 陕西理工学院专项科研基金项目(SLGQD0716)
【CateGory Index】: S632.9
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