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《Journal of Anhui Agricultural Sciences》 2010-23
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Genetic Diversity of Indocalamus Determined by Chloroplast DNA Sequence

MU Shao-hua et al (SFA Key Laboratory of Bamboo and Rattan Science and Technology,International Center for Bamboo and Rattan Beijing 100102)  
[Objective]The aim was to research affinity between Indocalamus and Sasa.[Method]Using 13 samples of Indocalamus and 3 samples of Sasa as materials,the intergenic regions of trnL-trnF gene in chloroplast were amplified by PCR,and sequence analysis and phylogenetic trees construction were carried out.[Result]Usigng the universal primer,the intergenic regions of trnL-trnF were amplified,the lengths of the segments varied from 1 008 bp to 1 103 bp,of which 940 bp was compared. The dendrogram of trnL-trnF sequences showed that Indocalamus and Sasa were clustered together and they were homologous by 99%. All the samples were divided into five groups,the first group included 12 sapmples such as Indocalamus pedalis,I. pumilus,I. victorialis,I. longiauritus,I. tessellatus,Sasa sinica,Sasa pygmaea,I. barbatus,I. guangdongensis,I. herklotsii,I. Hirtivaginatus and S. fortunei. I. decorus,I. lacunosus,I. Latifolius and I. Migoi were respectively divided into four groups. [Conclusion]The high homology of all samples showed the low evolution speed and little information sites which suggested that the phylogeny of Indocalamus could not be well resolved by the intergenic region of trnL-trnF.
【Fund】: “十一五”科技支撑项目(2006BAD19B0202);; 国际竹藤网络中心基本科研业务专项(1632009007);国际竹藤网络中心基金项目(06/07-C22)
【CateGory Index】: S795.9
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