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《Journal of Anhui Agricultural Sciences》 2010-24
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Extraction Technology of Compound Prescription of Traditional Chinese Medicine under Orthogonal Test

CHEN Deng et al (College of Veterinary Medicine,Sichuan Agricultural University,Yaan,Sichuan 625014)  
[Objective] The objective was to screen the optimal extraction technology of compound prescription of traditional Chinese medicine,which is composed of radix sophorae flavescentis,rhizoma smilacis glabrae and cortex dictamni,aiming at providing experimental evidence for the development and utilization of the compound prescription. [Method] With the minimal inhibitory concentration ( MIC ) of extracts for Candida albicans as index,the orthogonal test was designed with 4 factors:concentration of ethanol (A),extraction time (B),the proportion of three components (C) and material-liquid ratio (D) to screen the optimum extraction conditions. [Result] The optimum technology were as follows:extraction solution of 70% ethanol ( V/V ),proportion of 2:2:1,solid-liquid ratio of 1:10 and extraction time of 2 h,under which the yield of the compound prescription was 23% and the MIC value was 0.195 mg/ml. [Conclusion] The optimum extraction technology was obtained from the orthogonal test,and this will provide reference for the development and utilization for the compound prescription.
【Fund】: 教育部《长江学者和创新团队发展计划》创新团队项目(IR-TO848);; 宜宾市科学技术研究项目(2010C001)
【CateGory Index】: R284.2
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