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《Journal of Anhui Agricultural Sciences》 2010-26
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Study on the Extraction and Determination of Lentinan in Mushrooms

CHEN Yu-feng et al(Colledge of Chemistry and Chemical Engineering,Mudanjing Normal University,Mudanjiang,Heilongjiang 157012)  
[Objective] The aim was to study the extraction of the lentinan in mushrooms and determine the content of lentinan.[Method] The effection of different extraction conditions on extraction efficiency of lentinan in mushrooms by using hot water,microwave-assisted extraction and ultrasonic-assisted extraction methord,and trasonic-assisted extraction methord was the best methord.Then lentinan was extracted by trasonic-assisted extraction methord,and optimum conditions of the determination of lentinan content were studied by phenol-sulfuric acid methord to determine the lentinan content of northeast mushrooms.[Results] The linear equation was y=7.408 9c + 0.016 3,and the linear coefficient was 0.998 7,the precision was RSD2.00%(n=5).[Conclusion] Trasonic-assisted extraction methord was proved to be simple,rapid,accurate and the best extraction method.
【Fund】: 牡丹江师范学院指导项目(KZ2008008) 牡丹江师范学院重点项目(Z2008003)
【CateGory Index】: S646.12
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