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《Journal of Anhui Agricultural Sciences》 2010-32
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Extraction of Pumpkin Polysaccharide by Complex Enzyme Method and Its Antioxidant Research

SUN Jie et al(Biological Engineering Department,Henan College of Urban Constrction,Pingdingshan,Henan 467044)  
[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin.Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid ratio and pH value on the extraction yield of polysaccharide from pumpkin.[Method] The best enzyme ratio and extraction conditions for complex enzymes extraction were determined through orthogonal tests.Scavenging ·OH and O-2 activities of pumpkin polysaccharides were also investigated by salicylic acid and improved self-oxidation of o-pheno methods respectively.[Result] The results showed that the biggest extraction yield of polysaccharide from pumpkin can be got when adding 1% cellulose enzyme,1.5% pectinase,1.0% papain and Na2HPO4-citric acid buffer solution(pH was 4.6),and oscillating for 30 min under water at 40 ℃ with the solid-liquid ratio of 1∶30.In addition,pumpkin polysaccharides had a strong activity of eliminating oOH,but very weak activity to scavenge O-2.[Conclusion] This study provided basic data for research and application of Pumpkin polysaccharide.
【Fund】: 河南省科技计划重点科技攻关项目(102102110157);; 河南城建学院科学研究基金项目(2010JZD008)
【CateGory Index】: S642.1
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