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《Journal of Anhui Agricultural Sciences》 2010-32
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Study on Ecological Distribution and Antimicrobial Activity of Actinomycete from Soil in the Mt. Everest National Nature Preserve

HE Jian-qing et al (Tibet Agricultural and Animal Husbandry College,Linzhi,Tibet 860000)  
[Objective]In order to explore the ecological distribution and antibacterial activity of soil actinomycete in Everest Nature Reserve,which could also provide microbial resources to screen new drug lead compounds.[Methods] Soil actinomyces of Everest Nature Reserve were isolated and antagonistic determination by dilution method of slab and Agar-agar method.[Results]The number of actinomycetes distribution was significantly different in different vegetation types in Everest Nature Reserve.Farmland soil contained a greater number of actinomyces,the soil of plantations and the original forest soil contained the least amount.In the same soil profile,the number of the actinomyces on the layer of litter was the greatest,the deeper the soil,the less the organic matter and the fewer the number of actinomyces.The soil actinomycetes flora were complex and 10 genera were found.Streptomyces occupied an absolutely dominant position,and could be classified into 11 groups,Albacores,Griseofuscus and Roseosporus were dominant groups.Determination of antagonistic actinomycetes illustrated that the tested strains had stronger antimicrobal activity against fungi than bacteria,had stronger antimicrobal activity against Gram positive bacteria than Gram-negative bacteria in Everest Natural Reserver.[Conclusion]Everest Nature Reserve implied rich and strong antagonistic activity of actinomycetes.The strains showed high antimicrobial activities,and could be further researched for exploiting new compounds of pharmaceutical.
【Fund】: 国家自然科学基金项目(3076005)
【CateGory Index】: S476;S154.3
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