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《Journal of Anhui Agricultural Sciences》 2010-34
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Determination of Contents of Hypericin in Hypericum perforatum L.by HPLC

WANG Xiu-ying (Jilin Agricultural Science and Technology College,Jilin,Jilin 132101)  
[Objective]To determine the content of hypericin in Hypericum perforalurn L.by HPLC,so as to provide references for its development and utihzation.[Method]The content of hypericin was determined by HPLC,and HPLC conditions were as follows: column of SHIMADZU VP-ODS C18 column,mobile phase of methanol-ethyl acetate-phosphate buffer(V∶V=60∶20∶20,pH=2.0),flow rate of 1.0 ml/min,column temperature of 25 ℃,detection wavelength of 590 nm and injecting amount of 10 μl.[Result]In the range of 2.56-19.20 μg/ml,the linear relationship of hypericin was good(r=0.999 9).The content of hypericin in Hypericum perforalurn L.was 0.79 mg/g and the yield of it was 0.079%.[Conclusion]The method established in this study with good precision,reproducibility and stability is favorable for the determination of hypericin in Hypericum perforalurn L..
【Fund】: 吉林省教育厅“十一五”规划重点项目(2007238)
【CateGory Index】: R284
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