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《Journal of Anhui Agricultural Sciences》 2010-34
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Screening and Application of SSR Markers of Resistance Gene against Rice Stripe Virus

CAI Zhi-jun et al(Jiaxing Academy of Agricultural Sciences,Zhejiang Province,Jiaxing,Zhejiang 314016)  
[Objective]New SSR primers were designed and screened to apply in the backcross breeding for modified resistance against rice stripe virus.[Method]The conventional late japonica rice varieties including 502 with high resistance to stripe virus,Xiushui09 with high susceptibility to stripe virus and their derived strains were adopted as the test material,SSR and SAPR markers was used to locate RSV1 gene with high resistance against stripe virus,and three pairs of SSR markers(M-11-1,M-11-2,M-11-3) were further designed.Through screening and analysis,M-11-3 was selected as the RSV1 detection marker gene for tracking RSV1 gene,thus RSV1 gene was successfully introduced to the backcross breeding of late japonica rice varieties such as Xiushui 09,and the resistance expression of different strains was identified.[Result]The resistance of improved strains against stripe virus was significantly higher than Xiushui 09,the resistance of most strains was close to the level of donor,and the expression of resistance among years was stable.Therefore,the resistance effect of RSV1 gene used in the test was very obvious,which was accurate with the assisted selection of RSV1 gene linked markers M-11-3.[Conclusion]The study certified the feasibility of molecular markers application in resistance improvement against rice stripe virus,which also showed that optimization and development of new marker genes could effectively improve the efficiency of marker-assisted selection.
【Fund】: 浙江省重大项目(2006E10053);; 国家科技支撑计划项目(2006BAD01A01-5)
【CateGory Index】: S435.111.4
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