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《Journal of Anhui Agricultural Sciences》 2011-01
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Modified One Step Isolation of RNA,DNA and Protein from Plant Tissues

XIAO Xiao et al (College of Life Sciences,Nanjing Agricultural University,Nanjing,Jiangsu 210095)  
[Objective] The aim of this study was to extract RNA,DNA and protein from plants by using modified one step method. [Method] CTAB was used for the modifying of Biozol-based one step extraction method,which was then used for the isolation of RNA,DNA and protein from the roots of maize(Zea mays L.) ,soybean(Glycine max L.) ,alfalfa(Medicago sativa L.) and cucumber(Cucumis sativus L.) . And the heme oxygenase-1 gene(HO-1) and calcium-dependent protein kinase-1 gene(CDPK1) ,as well as HO-1 protein were identified. [Result] The result of UV spectrophotometer determination and agarose gel electrophoresis analysis showed that the purity of obtained RNA and DNA sample was relatively high;HO-1 and CDPK1 gene exhibited the positive signals checked by RT-PCR;the extracted protein could be used for western blotting analysis;the whole isolation procedure cost 3 h. [Conclusion] This method can be used as a good reference for researcher and will offer a better value with a wide range of applications.
【Fund】: 农业部橡胶树生物学重点开放实验室/省部共建国家重点实验室培育基地-海南省热带作物栽培生理重点实验室开放课题(KLOF0907);; 转基因生物新品种培育重大专项(2009ZX08012-014B);; 国家自然科学基金项目(30971711);; 江苏省自然科学基金项目(BK2009309)
【CateGory Index】: S184
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【Citations】
Chinese Journal Full-text Database 7 Hits
1 ZHANG Zheng-yan,LIU Peng,CHEN Wei-wei,CHEN Chuan-qi,JIA Jia Laboratory of Biological Science,Zhejiang Normal University,Jinhua 321004,China;FTIR Study of the Endurance to Al of Different Soybean Cultivars[J];Spectroscopy and Spectral Analysis;2009-02
2 YANG Yuhong~(1,2),LIANG Dongchun~(1),GUO Gang~(1),ZHANG Jingyu~(1)~(1)Institute of Endocrinology,Metabolic Disease Hospital of Tianjin Medical University,Tianjin,300070,China~(2)Department of Biochemistry,Tianjin Medical University,Tianjin,300070,China;Modified Method for Simultaneous Isolation of Genome DNA with TRIZOL Reagent[J];Journal of Medical Molecular Biology;2006-02
3 HUANG Xue-mei,ZHANG Shou-tao*,YANG Chao,WANG Fang(Department of Bioengineering,Zhengzhou University,Zhengzhou 450001,China);Comparison of Different Methods for Total RNA Extraction from Maize Pollen[J];Journal of Henan Agricultural Sciences;2009-04
4 ZHOU Jian-chang1,YANG Ming-jie1,YANG Xing-fen1,HUANG Jun-ming1,LING Wen-hua2, HUANG Qiong1,LI Wen-li1 (1.Institute of Toxicology, Guangdong Center of Disease Control and Prevention, Guangzhou 510300, China; 2.Department of Nutritional Science, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China);DNA Extraction from Genetically Modified Foods by Modified CTAB Method or SDS Method[J];Food Science;2005-02
5 SHEN WenBiao,Wang Ren,WANG YiHua,ZHENG TianQing,WAN JianMinesearch Institute,Nanjing Agricultural University,Nanjing 210095,China);A Novel Method of Extracting Total RNA from Rice Embryo Samples[J];Hereditas(Beijing);2003-02
6 WANG Yu-feng,WANG Qing-xiang,SHANG Li-wei(College of Agronomy,Shenyang Agricultural University,Shenyang 110161,China);Effects of Calcium on Root Activity and the Content of Osmotic Adjustable Organic Substances of Maize Seedlings Under NaCl Stress[J];Journal of Maize Sciences;2008-02
7 HE Qing-yuan1,2,WU Ping1,LI Zheng-peng1,ZHANG Xiao-hong1,HU Qin1(1.College of Life Science,Anhui Science and Technology University,Fengyang 233100,China;2.Soybean Research Institute,Nanjing Agricultural University,National Center for Soybean Improvement,National Key Laboratory for Crop Genetics and Germplasm Enhancement,Nanjing 210095,China);Comparative Study on of Extraction Methods of Total RNA from Medicago sativa[J];Chinese Journal of Grassland;2009-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Yuan et al(College of Life Science and Technology,Gansu Agricultural University,Lanzhou,Gansu 730070);Protective Effects of NO and H_2O_2 on Seed Germination and Seedling Growth of Horsebean under Cd Stress[J];Journal of Anhui Agricultural Sciences;2009-11
2 HUANG Jun-ming,LI Wen-li,HU Shuai-er,et al.Center for Disease Control and Prevention of Guangdong Province,Guangzhou 510300,China;Establishment and application of TaqMan PCR for detection of genetically modified soybean[J];South China Journal of Preventive Medicine;2008-05
3 ZHAO Wei-wei et al (Agricultural College of Yanbian University,Longjing,Jilin 133400);Study on Methods of Extracting Total RNA from Grape Inflorescence[J];Journal of Anhui Agricultural Sciences;2009-32
4 TAN Lili1,YAN Zhengmin1,XU Yaying1,YANG Jifeng2,WANG Yong1(1.College of Horticulture,Northeast Agricultural University,Harbin 150030,China;2.Heilongjiang Research Institute of Fenghe Maize,Shuangcheng Heilongjiang 150100,China);Comparison with total RNA extraction methods of tomato leaves[J];Journal of Northeast Agricultural University;2010-04
5 LI Yan,WANG Tie-mei,LU Xin-shi(Grassland Resources and Ecology Laboretry,Beijing Forestry University,Beijing 100083,China);Comparative study on extraction methods of total RNA from creeeping-rooted alfalfa[J];Pratacultural Science;2010-05
6 WU Cheng-jiu et al(Bioenergy and Biomaterial Research Center,Institute of Life Science and Technology,Hunan University,Changsha,Hunan 410082);Extraction Methods of Total RNA from Houttuynia cordata Leaves[J];Journal of Anhui Agricultural Sciences;2010-29
7 ZHANG Xiao-juan et al(School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong,Shaanxi 723001);Comparative Study on Extraction Method for Total RNA from Leaves of Dioscorea opposita[J];Journal of Anhui Agricultural Sciences;2010-33
8 GAO Hua-Na HAO Xue-Juan GUAN Ying(College of Science,Yanshan University,Qinhuangdao,Hebei 066004,P.R.China);Fast Determination of Main Components of Five Kinds of Soybean by Fourier Transform Infrared Spectroscopy[J];Chinese Journal of Spectroscopy Laboratory;2011-01
9 YAN Hong-bo,LI Gui-qin (Bioscience and Bioengineering School, Hebei University of Economics and Business, Shi Jiazhuang 050061, China.);A High-efficient Protocol to Isolate Total RNA from Endosperm during Middle and Late Development Stage[J];Hubei Agricultural Sciences;2009-03
10 YANG Yin-gui1,2,ZHUANG Yong1,LOU Qun-feng1,LIU Qiang1,CAO Qing-he1,CHEN Jin-feng1* (1.State Key Laboratory of Plant Genetics and Germplasm Enhancement,NAU,Nanjing 210095,China;2.College of Agronomy,JAU,Nanchang 330045,China);A Quick and High Efficient Method for Isolation of RNA from Young Cucumber Leaves[J];Acta Agriculturae Universitatis Jiangxiensis;2007-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Du Xihua; Lu Hai; Gao Shumin; Li Fenglan. School of Biological Science and Biotechnology, Beijing For. Univ., 100083, P. R. China.;Method for RNA isolating from anthers of Xanthoceras sorbifolia Bunge.[J];Journal of Beijing Forestry University;2003-01
2 WANG Yan-hua,WANG Cheng-zhang,SHI Ying-hua,LI Zhen-tian,CHEN Ji-hong,PAN Jun-liang(Engineering College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China);The research progress on alfalfa polysaccharides[J];Pratacultural Science;2007-04
3 JIANG Yi-bao~1,CUI Guo-wen~2,LI Hong~3(1.College of Animal Science,Henan Agriculture University,Zhengzhou 450002,China;2.College of Animal Science,Northeast Agriculture University,Haerbin 150030,China;3.Animal Science Institute of Heilongjiang Province,Qiqihar 161041,China);Effect of coating Medicago sativa seeds in calcium on drought resistance and interrelated physiological indexes under drought conditions[J];Acta Pratacultural Science;2005-05
4 Zhao Xiu-ying and Zhang Hong-li ( Northwest Institute of Botany, Shaanxt Yangling );STUDY ON CONSTITUENTS OF POLLEN OF Zea mays L.[J];Acta Botanica Boreali-occidentalia Sinica;1989-01
5 CAO Rang~1, LIANG Zong-suo~(1,2), WU Yong-jun~1, ZHANG Lin-sheng~1, KANG Shao-zhong~3 (1.College of Life Science, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China; 2.Institute of Soil and Water Conservation, CAS, Yangling, Shaanxi 712100, China; 3.College of Water Conservancy and Architectural Enqineering, Northwest Sci-Tech University of Agriculture and Foresty, Yangling, Shaanxi 712100, China);The change of amino acids in leaves of maize seedlings under alternative split-root osmotic stress[J];Agricultural Research in the Arid Areas;2004-01
6 Zhang Jianxia Li Xinguo Sun Zhonghai(Citrus Research Institute, Huazhong Agricultural University, Wuhan 430070,China);Effects of Exogenous Calcium on the Correlative Physiological and BiochemicalIndex on Heat-resistance of Citrus[J];Journal of Huazhong Agricultural;2005-04
7 FAN Hong-hong, LI Ting-chun, LIN Yi*, CAI Yong-ping(College of Life Science, Anhui Agriculture University, Hefei 230036, Anhui, China);The Method of RNA Isolation from Dendrobium[J];Acta Laser Biology Sinica;2007-01
8 FU Zuo shen, LI Shu kai, HUANG Shi ling, DU Juan, MA Jian jun (PLA Dalian Junior College of Medicine, Dalian 116017, China);Analysis of Nutritional Component of Maize Pollen[J];Journal of Clinical Medical officer;2000-04
9 FENG Jian-rong1, 2, CHEN Xue-sen1**, Wu Yan1 (1. Biological Laboratory of Pomology, Shandong Agricultural University, Tai'an 271018, China; 2. Horticultural Department, Shihezi University, Shihezi 832003, China);Extraction of Total RNA from Prunus armeniaca Style[J];Journal of Agricultural Biotechnology;2006-05
10 GAO Hong-wei 1* ,CHEN Chang-fa 1,DONG Dao-feng 2 (1.Qingdao Entry-Exit Inspection and quarantine Bureau, Qingdao 266002, China; 2.Shandong Academy of Agricultural Sciences, Jinan 250001,China);DETECTION METHOD OF GENETICALLY MODIFIED POTATOES BY PLOYMERASE CHAIN[J];Journal of Shandong Agricultural University;2002-04
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