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《Journal of Anhui Agricultural Sciences》 2011-03
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Active Ingredients from Metabolite of Datura arborea Entophytic Fungi and Its Anti-dermal Pathogenic Fungi Activities

ZHENG Yi et al(School of Biological and Chemical Engineering,Panzhihua University;Dry-hot Valley Characteristic Bio-Resources Engineering Center of Panzhihua City,Panzhihua,Sichuan 617000)  
[Objective]Antibiotic activities of metabolite of entophytic fungi strain Ym311977 isolated from the Datura arborea and its active ingredients were studied.[Method] Ym311977 was large-scale cultured in the liquid PDA media,and anti-dermal pathogenic fungi activities in vitro of single compounds which were separated from ferment outcomes were tested.[Result] One of four compounds separated from ferment outcome could inhibit the growth of Microsporum gypseum and Trichophyton gypseum,minimum inhibitory concentration were 50,200 μg/ml respectively.Through the identification by 1HNMR and 13CNMR,the structure of single compound was 5-Butylpicolinic acid,named fusaric acid.[Conclusion] It was the first time reported that fusaric acid could inhibit the growing of Microsporum gypseum and Trichophyton gypseum which were isolated from the metabolite of Datura arborea entophytic fungi.
【Key Words】: Endophytic fungi Antibiotic activity Fusaric acid Pathogenic fungi
【CateGory Index】: R756;S182
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