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《Journal of Anhui Agricultural Sciences》 2011-13
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Study on the Commonality of Camellia sinensis EST-SSRs in Theaceae Plant

CHENG Xiao-mao et al(College of Landscape Architecture,Southwest Forestry University,Kunming,Yunnan 650224)  
[Objective] The research aimed to study the commonality of Camellia sinensis EST-SSRs in Theaceae plant.[Method] Seven pairs of EST-SSRs primers which derived from Camellia sinensis EST sequence were used to amplify the nineteen species in Theaceae plant.[Result] Five pairs in the seven pairs of EST-SSRs primers could effectively amplify the nineteen test materials,and the commonality rate was 71.43%.The amplification rate of Camellia retiacalate Fengshancha,Camellia japouica Caixia and Camellia retiacalate Juban was the highest,and the amplification rate of Camellia synaptica Sealy and Adinandra sagonica var.Wallichiana(oc) Ming was the lowest.Moreover,four pairs in the five pairs of primers which could effectively amplify showed the rich polymorphism whose difference was obvious in the test materials.[Conclusion] SSR primers which were developed from the tea plant genome had the higher commonality in the different varieties of Theaceae plant and could be used in the comparative genome research and analysis mark research of Theaceae plant.
【Fund】: “省部级重点学科、省高校重点实验室及校实验室共享平台”项目;; 西南林业大学校级重点基金项目(110909)
【CateGory Index】: S571.1
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