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《Journal of Anhui Agricultural Sciences》 2011-14
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Study on Antibacterial Activities of Crude Extracts from the Whole Herb of Orostachys cartilaginous A. Bor in Vitro

REN Rong et al(School of Life Science and Technology,Mudanjiang Teachers′ College,Mudanjiang,Heilongjiang 157012)  
[Objective] To study antibacterial activities of crude extracts from the whole herb of Orostachys cartilaginous A.Bor in vitro.[Method] liquid-liquid extraction technique was applied to isolate active ingredients from 0.75 acetone extracts for preparing chloroform,ethyl acetate,normal butanol and water phases.The antibacterial activities of extraction phases on 4 tested strains in vitro were investigated using the filter paper method and double dilution method.[Result] Extraction phases from 0.75 acetone extracts had antimicrobial activities against Staphyloccocus aureus Rosenbach,Bacillus subtilis(Ehrenberg) Cohn,Escherichia coli and Proteus vulgaris in varying degrees.Chloroform and ethyl acetate extraction phases were provided with strong antibacterial activities against Staphylocoaus aureus with minimal inhibitory concentration at 1.25 mg/ml.[Conclusion] Active ingredients of Orostachys cartilaginous A.Bor plant were mainly existed in chloroform and ethyl acetate phases,which could provide a scientific basis for developing antibacterial agents from Orostachys cartilaginous A.Bor.
【Fund】: 黑龙江省教育厅科学技术研究项目(11551514);; 牡丹江师范学院教学改革工程项目(10-XY01038)
【CateGory Index】: S567.239
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