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《Journal of Anhui Agricultural Sciences》 2011-15
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Research of Water Medium Domestication Technology in Rice Seedlings of Tissue Culture

CHEN Qiang et al(Experimental Center,Shenyang Normal University,Shenyang,Liaoning 110034)  
[Objective] The aim was to research water medium domestication technology in rice seedlings of tissue culture.[Method] Firstly,take some plastic beakers for 500 ml and add 1/4 times nutrient solution of Haogland(NAA 0.5 mg/L),then lid a cover with holes on the beakers.Secondly,take 3-5 seedlings from tissue culture and wrapped its stem base gently with sponge,then insert them into the holes of covers.Put the seedlings into the illuminating incubator to cultivate by adjusting temperature,humidity and light.[Result] Domestication seedlings of water medium had high survival rate.Chlorophyll content was not less than 1/4 of chlorophyll content of normal seedlings.DNA content was similar.[Conclusion] Water medium domestication was simple and labor-saving.Domestication cycle was short.Water medium domestication had high survival rate.Domestication seedlings could be used in the related physiology,biochemistry and molecular experiments.
【Fund】: 国家自然科学基金项目(30870205)
【CateGory Index】: S511
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 ZHENG Chunfang,JIANG Dong,DAI Tingbo,JING Qi,CAO Weixing Key Laboratory of Crop Physiology and Ecology in Southern China,Ministry of Agriculuture /Hi-Tech Key Laboratory of Information Agriculture,Jiangsu Province,Nanjing Agricultural University,Nanjing 210095,China;Effects nitroprusside,a nitric oxide donor,on carbon and nitrogen metabolism and the activity of the antioxidation system in wheat seedings under salt stress[J];Acta Ecologica Sinica;2010-05
2 YUAN Yun-xiang,WAN Zhi-gang,SUN Bing-yao(School of Life Sciences,Suzhou University,Suzhou Jiangsu 215123,China);Optimization on Plant Regeneration Method of Rice Callus[J];Seed;2008-01
3 Hui Chen1,2, Yuan Zhao1, Kang Chong1* 1Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 2College of Life Sciences, Shanxi Normal University, Linfen 041004, China;Improved High-efficiency System for Rice Transformation Using Mature Embryo-derived Calli[J];Chinese Bulletin of Botany;2008-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 YAO Fang-yin,ZHU Chang-xiang,LIU Li-mei,ZHOU Zhan-yun, LI Guang-xian (Shandong Rice Research Institute, Jining 272077, China);Study on improving embryogenic callus induction and plant regeneration rates from mature embryos in rice[J];SHANDONG AGRICULTURAL SCIENCES;2000-04
2 XU Ding-hua et al(College of Life Science and Engineering,Huanggang Normal University,Huanggang,Hubei 438000);Cryopreservation of Plant Germplasm Resources[J];Journal of Anhui Agricultural Sciences;2007-02
3 HEI Shu-mei et al(College of Life Science,Yanan University,Yanan,Shaanxi 716000);Effect of DNA Methylation on the Plant Growth and Development[J];Journal of Anhui Agricultural Sciences;2007-21
4 MENG Fan-rong et al(College of Life Science,Henan Agricultural University,Zhengzhou,Henan 450002);DNA Methylation and its Biological Role in Plant[J];Journal of Anhui Agricultural Sciences;2007-36
5 ZHAI Xiao-qiao et al (Key Laboratory of Forest Germplasm Resources Protection and Improved Variety Breeding of Henan Province,Zhengzhou,Henan 450008);Study on the Cryopreservation of Robinia pseudoacacia Seeds[J];Journal of Anhui Agricultural Sciences;2008-02
6 CUI Hai lan,ZHU Yan ming ** ,QI Yang (Plant Bioengineering Laboratory,Academy of Life Science,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Effect of thidiazuron on rice (Oryza sativa L.) callus organogenesis[J];Journal of Northeast Agricultural University;2003-01
7 WU Xue-xia~ 1,2 ,ZHU Yue-lin~1,ZHU Wei-min~ 2* ,CHEN Jian-lin~2(1 College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2 Horticultural Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai Key Laboratory of Protected Horticultural Technology,Shanghai 201106,China);Effects of Exogenous Nitric Oxide on Seedling Growth of Tomato under NaCl Stress[J];Acta Botanica Boreali-Occidentalia Sinica;2006-06
8 LI Guo-liang (Academy of Agricultural Science, Harbin 150086, China);Effect of Cadmium on maize seeds germination and seedling growth[J];Territory & Natural Resources Study;2006-02
9 Ge Taiming; Yu Yujun(Huazhong Agricultural University, Wuhan 430070);EFFECTS OF GENOTYPES AND MEDIA ON WHEAT ANTHER CULTURE[J];JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY;1996-05
10 NIU Chuan-po1, JIANG Jing-yan1, HUANG Yao1,2 (1.College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; 2.Institute of Atmospheric Physics, China Academy of Sciences, Beijing 100029, China);Influence of Increased UV-B Radiation on C and N Metabolism of Winter Wheat[J];Journal of Agro-Environment Science;2007-04
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