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《Journal of Anhui Agricultural Sciences》 2011-15
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Research of Water Medium Domestication Technology in Rice Seedlings of Tissue Culture

CHEN Qiang et al(Experimental Center,Shenyang Normal University,Shenyang,Liaoning 110034)  
[Objective] The aim was to research water medium domestication technology in rice seedlings of tissue culture.[Method] Firstly,take some plastic beakers for 500 ml and add 1/4 times nutrient solution of Haogland(NAA 0.5 mg/L),then lid a cover with holes on the beakers.Secondly,take 3-5 seedlings from tissue culture and wrapped its stem base gently with sponge,then insert them into the holes of covers.Put the seedlings into the illuminating incubator to cultivate by adjusting temperature,humidity and light.[Result] Domestication seedlings of water medium had high survival rate.Chlorophyll content was not less than 1/4 of chlorophyll content of normal seedlings.DNA content was similar.[Conclusion] Water medium domestication was simple and labor-saving.Domestication cycle was short.Water medium domestication had high survival rate.Domestication seedlings could be used in the related physiology,biochemistry and molecular experiments.
【Fund】: 国家自然科学基金项目(30870205)
【CateGory Index】: S511
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