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《Journal of Anhui Agricultural Sciences》 2011-15
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Isolation and Preliminary Identification of Antimicrobial Peptides Produced by Antagonistic Actinomycetes against Peach Crown Gall(Agrobacterium tumefaciens)

JI Jing-lin et al(Department of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206)  
[Objective] The paper was to isolate and preliminarily identify the antimicrobial active substances of antagonistic actinomycetes strain G19 obtained from the soil highly affected by peach crown gall(Agrobacterium tumefaciens).[Method] The antimicrobial substances of antagonistic actinomycetes strain G19 were extracted using protein precipitation method,then isolated and purified using high performance liquid chromatography and middle-pressure chromatography.Its molecular weight was determined by MALDI-TOFMS method,and the related functional groups were verified through chemical color reaction.[Result] Seven peptide portions were produced from the antimicrobial substances of antagonistic actinomycetes strain G19 after isolation and purification with the molecular weights of 900-1 300 Da.It could be also inferred that it contained Cys,and carried with H2O and Na+.Color reaction of functional groups verified that the substance was polypeptide containing glycosyl.[Conclusion] The result provided basis for the final definition of the structure of antimicrobial substances in antagonistic actinomycetes strain G19.
【Fund】: 北京市教委资助项目(KM200910020001);; 北京市自然科学基金(5112010)
【CateGory Index】: S436.621
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