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《Journal of Anhui Agricultural Sciences》 2011-16
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Effects of GA on Germination of Corydalis yanhusuo W.T.Tang and Effect of NO on Its Adventitious Root Generation

ZHANG Yuan-hua(Department of Life Science,College of Chemistry and Life Sciences,Weinan Teachers College,Weinan,Shaanxi 714000)  
[Objective] To investigate effect of GA on germination of Corydalis yanhusuo W.T.Tang,and to discuss influence of NO on adventitious root generation.[Method] Using tuber of Corydalis yanhusuo W.T.Tang as material,germination test of Corydalis yanhusuo W.T.Tang treated with GA of different concentration was carried out at different temperature to investigate effect of GA.With NADPH-diaphorase as a marker for NOS,effect of NO on generation of adventitious root was discussed.[Result] At 4 and 16 ℃,Corydalis yanhusuo W.T.Tang treated with GA of different concentration was better in germination,while it could not germinate at 22 ℃ due to decayed tuber.First of all,Corydalis yanhusuo W.T.Tang treated with 0.6 mg/L GA at different temperature was highest in germination rate.The NOS-like enzyme mainly existed in the tissue of adventitious roots and the positive reactions mainly centralized in the root meristem,especially in the tip of root anlage.The positive reactions were full of the whole cytoplast.[Conclusion] GA played a positive role in the germination of Corydalis yanhusuo W.T.Tang,and NO appeared to playa key role in the generation and development of adventitious root.
【Fund】: 陕西省自然科学研究计划基金项目(2003C01);; 渭南师范学院科研资助项目(11YKZ005)
【CateGory Index】: S567.239
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