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《Journal of Anhui Agricultural Sciences》 2011-18
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Observation of the Stomatal Behavior of Test-tube Plantlet of Transgenic Male Abortive Populus tomentosa Carr

YANG Jin-hua et al(College of Forestry,Agricultural University of Hebei,Baoding,Hebei 071000)  
[Objective]To study the causes of abnormal stomatal behavior of test-tube plantlet.[Method] The change of stomatal density,stomatal size,the daily variation of stomatal aperture and stomatal open percentage of the leaves' lower epidermis of test-tube plantlets and 10-year-old plants of transgenic male abortive Populus tomentosa Carr were observed and compared in this paper by using nail polish method.[Result] The stomatal density of test-tube plantlets was less than that of the 10-year-old plant;test-tube plantlets' stomata was obviously larger than that of the 10-year-old plant;the stomatal aperture of test-tube plantlets showed wave crest,and the two peaks appeared at 9:00 and 15:00,10-year-old plant had steady curve without evident fluctuation,and the stomatal aperture in test-tube plantlets was greater than that in the 10-year-old plant;stomatal open percentage reached 100% in test-tube plantlets during a day,a bimodal curve change was observed in stomatal open percentage of the 10-year-old plant.[Conclusion] Stomatal behavior were different between the test-tube plantlets and the 10-year-old plant of transgenic male abortive Populus tomentosa Carr.
【Fund】: 河北省林业局资助项目(0207216)
【CateGory Index】: S792.117
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