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《Journal of Anhui Agricultural Sciences》 2011-20
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Preliminary Study on Immunocompetence of Polysaccharides from Pleurotus citrinopileatus Sing

LU Yu-lan et al (College of Life Science,Langfang Teachers College,Langfang,Hebei 065000)  
[Objective] To study the extraction method of polysaccharides from Pleurotus citrinopileatus Sing and its effects on immune function in animals.[Methods] Through the liquid culture,the polysaccharides were extracted from fermentation broth of Pleurotus citrinopileatus Sing by ethanol precipitation method,whose extraction rate was determined by combining anthrone colorimetry technology with 3,5-dinitrosalicylic acid method.Effects of different doses of polysaccharides from Pleurotus citrinopileatus Sing on immune function in mice were explored by weighing thymus and spleen and calculating thymus index and spleen index.[Results]The extraction rate of polysaccharides was 14.35%.With the dose of polysaccharides increasing,spleen weight gradually increased.The immunity in mice that were injected with polysaccharides was significantly higher than that were injected with cyclophosphamide(CY),and lower than that were injected with normal saline(NS).[Conclusion] The polysaccharides from Pleurotus citrinopileatus Sing could play a role in CY attenuation and enhancing immune function in mice.
【Fund】: 2007年河北省第二批科学技术研究与发展指导计划(No.07225541);; 廊坊师范学院科学研究项目(LSZZ200802)
【CateGory Index】: S567.3
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