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《Journal of Anhui Agricultural Sciences》 2011-21
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Screening and Identification of Enterobacter Producing Succinic Acid in Soil

ZHOU Wen-yu et al(Food Science and Engineering,Jilin Agricultural University,Changchun,Jilin 130118)  
[Objective]The bacterial strain that could highly produce succinate acid and be with low requirement to nutrient condition was isolated from soil.[Method]The bacterial strain producing succinate acid was screened and isolated based on the accumulated medium combined with Rapid screening plate of bromocresol green.The strains with the different abilities in the production of succinate acid were separated with TLC method and fluorescence method and the qualitative and quantitative test of their ability were future measured with the HPLC analysis method.[Results] The morphologic characteristics of the strain and its colony appeared that the colony was viscous orbicular,convex round with smooth surface,opaque,milk white in color,jagged and irregular edge;and most of the cells appeared single or in pairs in cluster;the colony was facultativele anaerobic and negative in Gram reaction.The strain was assured to be Escherichia coli based on the further analysis of biochemical index and 16S rRNA sequence.[Conclusion]The yield of succinate acid from the strain-JLT9 was relevantly low but with few by-products,and also,its culturing condition was simple so that the strain had good prospect for its development and application.
【CateGory Index】: S154.3
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