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《Journal of Anhui Agricultural Sciences》 2011-24
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Isolation and Culture of Fetal Fibroblasts of Tibetan Yellow Cattle

REN Zi-li et al(Faculty of Animal Science,Tibet Agricultural and Animal Husbandry College,Linzhi,Tibet 860000)  
[Objective] To isolate fetal fibroblasts from Tibetan yellow cattle and thus to provide the ideal biological material for cloning and cellular biology of animals inhabiting in the Tibet Plateau.[Method] The fibroblast cells were isolated from Tibetan yellow fetus by explant culture method.The subculture,morphology and dynamics were observed.[Result] The fetal fibroblasts of Tibetan yellow cattle had typical morphology of fibroblast cells.The isolated cells had three growth stages including retention period(0-2 d),logarithmic phase(2-7 d) and platform phase(7-8 d).Its doubling time was 60 h.[Conclusion] Fetal fibroblasts of Tibetan yellow cattle have been successfully cultured.
【Fund】: 国家自然科学基金资助项目(31040077);; 211工程师资队伍建设项目资助(SZRC-211-01);; 西藏科技厅地区自然科学基金资助项目(2010-17-02)
【CateGory Index】: S823.1
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